Phenotypic and 90K SNP data for the wheat diversity panel of 385 accessions

Diversity Panel:

The panel includes a diverse collection of 385 accessions, encompassing 27 different species of cultivated wheats, synthetic hybrid wheat (SHWs), progenitor species and wild relatives were used in this study. The AB and ABD genomes are represented by 170 accessions representing T. vavilovii and several subspecies of T. turgidum and T. aestivum as well as 65 primary SHWs. The A, B and D genome progenitors (or their closely related species) and the non-domesticated forms of tetraploid wheat comprised 93 accessions of T. urartu (A), T. monococcum (A^m), Ae. tauschii (D), Ae. speltoides (S), as well as T. turgidum ssp. dicoccon and dicoccoides (AB). Another 47 accessions belonged to the following Aegilops species: Ae. bicornis (S^b), Ae. longissima (S^l), Ae. searsii (S^s), Ae. sharonensis (S^sh), Ae. markgrafii (C), Ae. comosa (M), Ae. umbellulata (U), Ae. geniculata (MU), Ae. peregrina (SU), Ae. triuncialis (UC/CU), Ae. columnaris (UM), Ae. cylindrica (DC), Ae. crassa (DM/DDM), Ae. juvenalis (DMU), Ae. biuncialis and Ae. neglecta (UM/UMN). The collection also contained six accessions of T. timopheevii (A^tG), five of T. zhukovskyi (GAA^m), and one of Haynaldia villosa (V), a related grass species. Overall, the germplasm consisted of 75 diploid, 136 tetraploid, 165 hexaploid and nine accessions that could be either tetraploid or hexaploid.

The panel contains 215 accessions of spring growth habit and 167 accessions of winter growth habit. In addition, there are five accessions that have both winter and spring growth habit.

The information of all accessions in the panel is included in the file:385_accessions_genetic_panel_info.XLSX.

Summary of accessions in the genetic panel

Phenotypic data:

Field evaluation:

Phenotyping of disease resistance and other agronomic traits was performed in separate field trials for the spring and winter panels. Trials for the spring and winter panels were carried out in several locations.

For each panel, year and location, a completely randomized design with two replicates was used, except for several trials.

LR race-specific response:

Consecutive inoculations with six P. triticina isolates were performed for 360 accessions of the panels. All tests were performed under controlled greenhouse conditions at the Morden RDC, AAFC (Morden, Canada). Briefly, test lines and the Thatcher and Emerson check lines were sown into fiber trays at a rate of approximately 5 seeds per clump and 3 cm between clumps, which were inoculated with individual P. triticina isolates at the two-leaf stage as described by McCallum and Seto-Goh (2006). The isolates tested were 12-3 MBDS, 128-1 MBRJ, 74-2 MGBJ, 11-180-1 TDBG, 06-1-1 TDBG, and 77-2 TJBJ, which represent the prevalent leaf rust race groups across Canada (McCallum et al., 2016). Virulence and avirulence formulas for these isolates are given. Infection type (IT) was rated 12 days post-inoculation using a 0–4 scale (Stakman et al., 1962), where “;” = hypersensitive flecks, “0” = no uredinia or macroscopic sign of infection, “1” = small uredinia with necrosis, “2” = small to medium uredinia with chlorosis, “3” = medium uredinia without chlorosis or necrosis, “4” = large uredinia without chlorosis or necrosis. IT “;” and “0” to “2” were considered resistant, while “3” and “4” were considered susceptible (Long and Kolmer, 1989). The “+” or “−” IT qualifiers indicate larger or smaller than average uredinia, respectively. The “ = ” IT qualifier represents the lower size limit of the uredinia for the IT (Long and Kolmer, 1989). Plants with randomly distributed uredinia of variable sizes, or mesothetic response, were considered resistant and were rated with an “X” IT (Roelfs and Martens, 1988).

Availability of phenotypic data in different years and locations

OTT: Ottawa, Ontario; MDN: Morden, Manitoba; SKT: Saskatoon, Saskatchewan; LTB: Lethbridge, Alberta; PEI: Prince Edward Island.
LR: leaf rust; FHB: Fusarium head blight; PM: powdery mildew; YR: yellow rust; VRI:visual rating index; DON: mycotoxin deoxynivalenol; FDK: Fusarium damaged kernels; TKW: thousand kernel weight; TSW: thousand seed weight;DM: days to maturity;YLD: yield per plot; HT: height; DH: days to heading.

Raw phenotypic data files

LR: leaf rust; FHB: Fusarium head blight; PM: powdery mildew; TWK: thousand kernel weight; TSW: thousand seed weight.
MDN: Morden, Manitoba; OTT: Ottawa, Ontario; SKT: Saskatoon, Saskatchewan, PEI: Prince Edward Island; LTB: Lethbridge, Alberta.

Processed phenotypic data files

LR: leaf rust; FHB: Fusarium head blight; VRI:visual rating index; DON: mycotoxin deoxynivalenol.
MDN: Morden, Manitoba; OTT: Ottawa, Ontario; SKT: Saskatoon, Saskatchewan.

SNP data of the wheat diversity panel (385 accessions).

SNP data files of 90K Chip Array for the 385 accessions in the diversity panel